DAF-FM - Enzo Life Sciences

ALX-620-070

Revised 01-Feb-10

DAF-FM
SYNONYMS	Diaminofluorescein-FM
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	Nitric Oxide & NOS Detection / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-070-M001		1 mg	570.00 USD

Product Specification

FORMULA:	C₂₁H₁₄F₂N₂O₅
MW:	412.3
CONCENTRATION:	~7mM
PURITY:	≥98% (HPLC)
FORMULATION:	Liquid. In 0.35ml DMSO.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Prepare 1’000-fold dilution (~7µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:	Keep under inert gas. After opening, prepare aliquots and store at -20°C. Protect from light.

Product Description

Highly sensitive fluorescent probe (Ex: 500nm; Em: 515nm) for the detection of nitric oxide (NO). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable. Not for sale in Japan.

Product Specific Literature References

Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem. 287, 203 (2000) Abstract
Fluorescent indicators for nitric oxide: H. Kojima & N. Nagano; Advanced Materials 12, 763 (2000)
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001) Abstract
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem. 76, 1404 (2001) Abstract