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DAR-4M

Fluorescent probe for NO detection
 
ALX-620-067-M001 1 mg 620.00 USD
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Fluorescent probe (Ex: ~560nm; Em: 575nm) for the detection of nitric oxide (NO). It can be used to detect NO directly in biopsy tissues, cultured cells, and tissue sections. Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM. Not for sale in Japan.

Product Specification

Alternative Name:Diaminorhodamine-4M
 
Formula:C25H26N4O3
 
MW:430.5
 
Concentration:~5mM
 
Formulation:Dissolved in 0.47ml DMSO.
 
Purity:≥98% (HPLC)
 
Appearance:Reddish-violet liquid.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
 
Handling:Warm to room temperature before opening. Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
 
ALX-620-067
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ALX-620-067

Product Literature References

Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001), Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & N. Nagano ; Advanced Materials 12, 763 (2000),

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RELATED PRODUCTS

By target:
Nitric oxide
By biological activity:
Nitric oxide Ligand
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