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DAF-2 DA (cell permeable)

Fluorescent probe for NO detection. Cell permeable.
 
ALX-620-056-M001 1 mg 458.00 USD
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Cell permeable analog of DAF-2 (Prod. No. ALX-620-052) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm).

Product Specification

Alternative Name:4,5-Diaminofluorescein . diacetate
 
Formula:C24H18N2O7
 
MW:446.4
 
CAS:205391-02-2
 
Formulation:Dissolved in 0.45ml DMSO.
 
Purity:≥98% (HPLC)
 
Appearance:Liquid.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
 
Handling:Protect from light. Keep under inert gas. Hygroscopic. After opening, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Replacement for ADI-906-042
 
620-056 new
daf-2-v2
Figure: The cell-permeable diacetate derivative, DAF-2 DA (Prod. No. ALX-620-056), is used to load cells. Subsequent hydrolysis by cytosolic esterases releases DAF-2 (Prod. No. ALX-620-052), which is relatively non-fluorescent at physiological pH. However, in the presence of NO and O2, DAF-2 is converted to the fluorescent triazole derivative, DAF-2T (Prod. No. ALX-620-060).
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620-056 new daf-2-v2

Product Literature References

Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice: A.M. Gamez-Mendez, et al.; PLoS One 10, e0138609 (2015), Application(s): In situ nitric oxide detection in mice, Abstract; Full Text
BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube: A. Traister, et al.; Cell Death Differ. 11, 832 (2004), Abstract;
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem. 277, 25823 (2002), Abstract; Full Text
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001), Abstract;
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001), Abstract;
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001), Abstract;
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000), Abstract;
Fluroescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000), Abstract;
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998), Abstract;
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998), Abstract;
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998), Abstract;
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998), Abstract;

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