Highly sensitive fluorescent probe for the real-time detection of nitric oxide (NO) in vivo. In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T, Ex 495nm; Em 515nm). Not for sale in Japan.
Product Details
| Alternative Name: | 4,5-Diaminofluorescein |
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| Formula: | C20H16N2O5 |
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| MW: | 364.3 |
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| CAS: | 205391-01-1 |
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| Concentration: | ~5mM |
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| Formulation: | Dissolved in 0.55ml DMSO. |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Pale yellow liquid. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions. |
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| Handling: | Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: The cell-permeable diacetate derivative, DAF-2 DA (Prod. No.
ALX-620-056), is used to load cells. Subsequent hydrolysis by cytosolic esterases releases DAF-2 (Prod. No.
ALX-620-052), which is relatively non-fluorescent at physiological pH. However, in the presence of NO and O2, DAF-2 is converted to the fluorescent triazole derivative, DAF-2T (Prod. No.
ALX-620-060)."
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Product Literature References
Hypoxic and nitrosative stress conditions modulate expression of myoglobin genes in a carcinogenic hepatobiliary trematode, Clonorchis sinensis: S.H.Kim, et al.; PLoS Negl. Trop. Dis.
15, e0009811 (2021),
Abstract;
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J.
15, 1291 (2001),
Abstract;
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab.
280, E171 (2001),
Abstract;
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett.
491, 227 (2001),
Abstract;
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol.
129, 1530 (2000),
Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC
274, 736 (2000),
Abstract;
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem.
70, 2446 (1998),
Abstract;
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull.
46, 373 (1998),
Abstract;
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett.
427, 263 (1998),
Abstract;
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport
9, 3345 (1998),
Abstract;
