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Fluorescent probe for NO detection
ALX-620-052-M001 1 mg 408.00 USD
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Highly sensitive fluorescent probe for the real-time detection of nitric oxide (NO) in vivo. In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T, Ex 495nm; Em 515nm). Not for sale in Japan.

Product Specification

Alternative Name:4,5-Diaminofluorescein
Formulation:Dissolved in 0.55ml DMSO.
Purity:≥98% (HPLC)
Appearance:Pale yellow liquid.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
Handling:Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Figure: The cell-permeable diacetate derivative, DAF-2 DA (Prod. No. ALX-620-056), is used to load cells. Subsequent hydrolysis by cytosolic esterases releases DAF-2 (Prod. No. ALX-620-052), which is relatively non-fluorescent at physiological pH. However, in the presence of NO and O2, DAF-2 is converted to the fluorescent triazole derivative, DAF-2T (Prod. No. ALX-620-060).
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ALX-620-052 daf-2-v2

Product Literature References

Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001), Abstract;
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001), Abstract;
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001), Abstract;
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000), Abstract;
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000), Abstract;
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998), Abstract;
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998), Abstract;
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998), Abstract;
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998), Abstract;

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