Bisbenzimide H33258 . trihydrochloride

DNA dye



ALX-620-051-M010	10 mg	14.00 USD

ALX-620-051-M050	50 mg	30.00 USD

Cell permeable fluorescent dye with low cytotoxicity. Useful for staining DNA, chromosomes and nuclei. Can be used with chromomycin A₃ (Prod. No. BML-GR302) to distinguish chromosomes by their total DNA concentration and their DNA base composition. Binds to A:T pairs in DNA. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles.

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Product Specification

Alternative Name:	HOE 33258, 2'-4-(Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole . 3HCl

Identity:	Identity determined by IR and ¹H-NMR.

Formula:	C₂₅H₂₄N₆O . 3HCl

MW:	424.4 . 109.4

Purity:	≥98% (TLC)

Appearance:	Yellow to greenish powder.

CAS:	23491-45-4

RTECS:	SM1140500

Solubility:	Soluble in water (10mg/ml).

Long Term Storage:	-20°C

Hazard:	HARMFUL. MAY BE MUTAGENIC.

Handling:	Protect from light.

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Product Literature References

Controlling gene expression in living cells through small molecule-RNA interactions: G. Werstuck & M.R. Green; Science 282, 296 (1998), Abstract;

Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations: K.H. Elstein & R.M. Zucker; Exp. Cell. Res. 211, 322 (1994), Abstract;

Activation and inhibition of the sarcoplasmic reticulum Ca2+ channel by the polycationic dyes Hoechst 33342 and Hoechst 33258: T.J. Beeler & K. Gable; J. Membr. Biol. 135, 109 (1993), Abstract;

Determination of guanine-plus-cytosine content of bacterial DNA by dual-laser flow cytometry: C.A. Sanders, et al.; J. Gen. Microbiol. 136, 359 (1990), Abstract;

Bacterial characterization by flow cytometry: M.A. Van Dilla, et al.; Science 220, 620 (1983), Abstract;

Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis: S.A. Latt & G. Stetten; J. Histochem. Cytochem. 24, 24 (1976), Abstract;

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