Bisbenzimide H33342 . trihydrochloride

DNA dye



ALX-620-050-M010	10 mg	14.00 USD

ALX-620-050-M050	50 mg	31.00 USD

Cell permeable fluorescent dye that binds to A:T pairs in DNA. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA. Induces cell differentiation. About 10 times more cell permeable than bisbenzimide H33258 (Prod. No. ALX-620-051). Ex(max): 346nm; Em(max): 460nm.

Product Specification

Alternative Name:	HOE 33342, 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole . 3HCl

Formula:	C₂₇H₂₈N₆O . 3HCl

MW:	452.6 . 109.4

Purity:	≥95%

Appearance:	Yellow to green powder.

CAS:	23491-52-3

Solubility:	Soluble in water.

Long Term Storage:	+4°C

Hazard:	MAY BE CARCINOGENIC. TOXIC.

Handling:	Protect from light.

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Product Literature References

Controlling gene expression in living cells through small molecule-RNA interactions: G. Werstuck & M.R. Green; Science 282, 296 (1998), Abstract;

A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells: F. Belloc, et al.; Cytometry 17, 59 (1994), Abstract;

Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis: S.A. Latt & G. Stetten; J. Histochem. Cytochem. 24, 24 (1976), Abstract;