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Amine dye
ALX-610-030-M025 25 mg 211.00 USD
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Non-toxic cell permeable fluorescent dye that reacts with amine compounds. Readily accumulates inside viable cells. Hardly leaks from cells when covalently attached to intracellular proteins. Hydrolyzed CFSE emits fluorescence (Ex(max): 495nm; Em(max): 519nm). Therefore, CFSE is utilized for the long-term observation of cell activities by fluorescent microscopy as well as for viable cell detection.

Product Specification

Alternative Name:5(6)-(N-Succinimidyloxycarbonyl)-3',6',0,0'-diacetylfluorescein, 5(6)-Carboxyfluorescein diacetate, N-succinimidyl ester-
Purity:≥95% (HPLC)
Appearance:White or slightly yellow powder.
Solubility:Soluble in DMSO, dimethyl formamide or methanol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Handling:Protect from light.
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Product Literature References

Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes: S.W. Kamau, et al.; BMC Pharmacol. 1, 1 (2001), Abstract;
Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question: E.J. Suchin, et al.; J. Immunol. 166, 973 (2001), Abstract;
Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution: A.B. Lyons; J. Immunol. Methods 243, 147 (2000), Abstract;
Flow cytometric cell division tracking using nuclei: J. Hasbold & P.D. Hodgkin; Cytometry 40, 230 (2000), Abstract;
Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro: H.E. Gruber, et al.; Biotech. Histochem. 75, 118 (2000), Abstract;
Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven: B.F. de St Groth, et al.; Immunol. Cell Biol. 77, 530 (1999), Abstract;
The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo: J. Mintern, et al.; Immunol. Cell Biol. 77, 539 (1999), Abstract;
Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester: A.B. Lyons; Immunol. Cell Biol. 77, 509 (1998), Abstract;
A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy: S. Oehen, et al.; J Immunol Methods 207, 33 (1997), Abstract;
Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function: L.S. De Clerck, et al.; J. Immunol. Methods 172, 115 (1994), Abstract;
New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy: S.A. Weston & C.R. Parish; J. Immunol. Methods 133, 87 (1990), Abstract;

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