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2',7'-Dichlorodihydrofluorescein diacetate

Peroxynitrite indicator
 
ALX-610-022-M050 50 mg 49.00 USD
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Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=59,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.

Product Specification

Alternative Name:DCDHF diacetate, 2',7'-Dichlorofluorescein diacetate
 
Formula:C24H16O7Cl2
 
MW:487.3
 
CAS:4091-99-0
 
Purity:≥95% (HPLC)
 
Appearance:White to off-white solid.
 
Solubility:25mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately dilute with PBS, pH 7.2, to the desired concentration.
 
ALX-610-022
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ALX-610-022

Product Literature References

Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999), Abstract;
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999), Abstract;
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997), Abstract;
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994), Abstract;

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