Dihydrorhodamine 123
[DHR]
| ALX-610-021-M005 | 5 mg | 153.00 USD |  |
|
Sensitive indicator of peroxynitrite formation. DHR is oxidized by peroxynitrite to the highly fluorescent product rhodamine. Formation of rhodamine can be monitored by fluorescence spectroscopy (Ex(max): 500nm, Em(max): 536nm), or by absorbance spectroscopy at 500nm (ε=78'800M-1cm-1). Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.
Product Specification
| Formula: | C21H18N2O3 |
| MW: | 346.4 |
| Purity: | ≥98% |
| Appearance: | Pink crystalline solid. |
| CAS: | 109244-58-8 |
| Solubility: | 10mg/ml soluble in 100% ethanol, DMSO or dimethyl formamide; sparingly soluble in PBS, pH 7.2 (20μg/ml). |
| Shipping: | AMBIENT |
| Long Term Storage: | -20°C |
| Use/Stability: | Stock solutions are stable for at least 6 months when stored at -20°C. We do not recommend storing aqueous solutions for more than one day. |
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Product Literature References
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994), Abstract;
Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen: K. Briviba, et al.; Biochem. J. 319, 13 (1996), Abstract;
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997), Abstract;
Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase: H. Sies, et al.; J. Biol. Chem. 272, 27812 (1997), Abstract; Full Text
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999), Abstract;
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999), Abstract;
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