Dihydrorhodamine 123 - Enzo Life Sciences

ALX-610-021

Revised 01-Feb-10

Dihydrorhodamine 123
SYNONYMS	DHR
PRODUCT LINE	Dyes / Stains / Fluorescent Probes
PRODUCT CATEGORY	Mitochondrial Staining

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-610-021-M005		5 mg	153.00 USD

Product Specification

FORMULA:	C₂₁H₁₈N₂O₃
MW:	346.4
CAS NUMBER:	109244-58-8
PURITY:	≥98%
APPEARANCE:	Pink crystalline solid.
SOLUBILITY:	10mg/ml soluble in 100% ethanol, DMSO or dimethyl formamide; sparingly soluble in PBS, pH 7.2 (20μg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for at least 6 months when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description

Sensitive indicator of peroxynitrite formation. DHR is oxidized by peroxynitrite to the highly fluorescent product rhodamine. Formation of rhodamine can be monitored by fluorescence spectroscopy (Ex(max): 500nm, Em(max): 536nm), or by absorbance spectroscopy at 500nm (ε=78'800M^-1cm^-1). Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.

Product Specific Literature References

Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994) Abstract
Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen: K. Briviba, et al.; Biochem. J. 319, 13 (1996) Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997) Abstract
Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase: H. Sies, et al.; J. Biol. Chem. 272, 27812 (1997) Abstract; Full Text
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999) Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999) Abstract

Further Categories Containing This Product:

Peroxynitrite / Scavengers / Detection • Respiratory Chain Other Products