| ALX-581-210-M001 | 1 mg | 450.00 USD |  |
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| Alternative Name: | Trehalose 6,6’-dimycolate, TDM, 6,6’-Di-O-mycoloyl-α,α-trehalose |
| Source: | Isolated from M. tuberculosis. |
| CAS: | 61512-20-7 |
| Formulation: | Lyophilized. |
| Purity: | Single spot (TLC) |
| Endotoxin Content: | <0.0002EU/µg (LAL test; BioWhittaker) |
| Solubility: | Soluble in chloroform:methanol:water (90:10:1) (5mg/ml), hexane or isopropanol. |
| Shipping: | Ambient Temperature |
| Long Term Storage: | +4°C |
| Protocol: | In vitro stimulation of macrophages Purified Cord Factor was used to stimulate either mouse RAW 264.7 cells or bone marrow-derived macrophages. Cord Factor was suspended at a concentration of 1mg/ml in isopropanol and sonicated in a bath sonicator for 5 min. This suspension was incubated at 60°C for 10 min. and sonication repeated. The resulting solution was layered onto 24-well tissue culture plates at the indicated concentrations and incubated at 37°C in order to ensure complete evaporation of the solvent. Control wells were layered with solvent without Cord Factor and incubated at 37°C. To this layer of Cord Factor, either RAW 264.7 cells or bone marrow-derived macrophages were added at a concentration of 106 cells in 100µl of medium and incubated at 37°C for 24 hours before activation e.g. TNF-α production was measured in the supernatant. Alternatively, Cord Factor was suspended at a concentration of 0.2 or 2mg/ml in hexane. Of the resulting solution 50µl were layered onto 96-well tissue culture plates at the indicated concentrations of 1 or 10µg/well, respectively and the solvent completely evaporated. Control wells were layered with solvent without Cord Factor and incubated at 37°C. To this layer of Cord Factor, either RAW 264.7 cells or bone marrow-derived macrophages were added at a concentration of 106 cells in 100µl of medium and incubated at 37°C for 24 hours before activation e.g. TNF-α production was measured in the supernatant. In vivo pulmonary granuloma formation in mice For in vivo experiments 10µg Cord Factor per mouse were applied i.v. in a water/oil/water emulsion. |
| Regulatory Status: | RUO - Research Use Only |
Monophosphoryl Lipid A from Salmonella minnesota R595 (Re) (TLRGRADE®) (Ready-to-Use) |
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| TLR4 activator | ||||||
| Isolated from Salmonella minnesota R595 (Re mutant). Prepared by acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF | ||||||
| ALX-581-202-R500 | 500 µl | 392.00 USD | |
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| ALX-581-202-L001 | 1 ml | 539.00 USD | |
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Monophosphoryl Lipid A from E. coli, Serotype R515 (Re) (TLRGRADE®) (Ready-to-Use) |
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| TLR4 activator | ||||||
| Isolated from E. coli, Serotype R515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, Serotype R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water., ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) | Print as PDF | ||||||
| ALX-581-203-R500 | 500 µl | 353.00 USD | |
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| ALX-581-203-L001 | 1 ml | 522.00 USD | |
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