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Lipid A from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)

TLR4 activator
 
ALX-581-201-L002 2 ml 389.00 USD
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Product Specification

Source:Isolated from Salmonella minnesota R595 (Re mutant). Prepared by mild acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
 
Purity:≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
 
Activity:Activates Toll-like receptor (TLR) 4. Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Handling:Keep sterile.
 

Product Literature References

CD14 is required for MyD88-independent LPS signaling: Z. Jiang, et al.; Nat. Immunol. 6, 565 (2005), Abstract;
IL-12 family members: differential kinetics of their TLR4-mediated induction by Salmonella Enteritidis and the impact of IL-10 in bone marrow-derived macrophages: N. Schuetze, et al.; Int. Immunol. 17, 649 (2005), Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;

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