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Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)

TLR4 activator
 
ALX-581-200-L001 1 ml 248.00 USD
 
ALX-581-200-L002 2 ml 405.00 USD
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Product Specification

Source:Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
 
Purity:≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
 
Activity:Activates Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Handling:Keep sterile.
 
lps
Figure: Activation of macrophages from TLR4 wild type compared to TLR4 deficient mice by LPS and Lipid A from ALEXIS. Lipid A or LPS concentrations, which induced maximal activation of  TLR4 wild type mouse macrophages, were also applied to TLR4 deficient mouse macrophages. 10 units of IL-6 correspond to the detection limit of the IL-6 ELISA.
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lps

Product Literature References

Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death: J. Shi, et al.; Nature 526, 660 (2015), Application(s): Cell culture, Abstract;
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;
R-form LPS, the master key to the activation of TLR4/MD-2-positive cells: M. Huber, et al.; methods in carbohy 36, 701 (2006), Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;

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