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LPS from Salmonella minnesota R5 (Rc) (TLRGRADE™) (Ready-to-Use)

TLR4 activator
 
ALX-581-017-L002 2 ml 198.00 USD
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Product Specification

Alternative Name:Lipopolysaccharide from Salmonella minnesota R5 (Rc)
 
Source:Rough (R)-form LPS isolated and purified from Salmonella minnesota R5 (Rc mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
 
Purity:Absence of detectable protein or DNA contaminants with agonistic TLR activity.
 
Activity:Strong activator of Toll-like receptor 4 (TLR4). Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required.Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at +4°C.
 
Handling:Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
 

Product Literature References

Structural definition of hSP-D recognition of Salmonella enterica LPS inner core oligosaccharides reveals alternative binding modes for the same LPS: J.R. Littlejohn, et al.; PLOS One 13, e0199175 (2018), Abstract; Full Text
R-form LPS, the master key to the activation of TLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006), Abstract;
Lipopolysaccharide regions involved in the activation of Escherichia coli outer membrane protease OmpT: R.A. Kramer, et al.; Eur. J. Biochem. 269, 1746 (2002), Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract;
Newer aspects of the chemistry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component: C. Galanos et al.; Int. Rev. Biochemistry 14, 239 (1977),
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;

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