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LPS from E. coli, Serotype O55:B5 (TLRgrade™) (Ready-to-Use)

Specific TLR4 activator
 
ALX-581-013-L001 1 ml 148.00 USD
 
ALX-581-013-L002 2 ml 210.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-581-001

Product Specification

Alternative Name:Lipopolysaccharide from E. coli, Serotype O55:B5 S-form
 
Source:Smooth (S)-form LPS isolated and purified from E. coli 055:B5 by a modification of the phenol water extraction and PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
 
Purity:Absence of detectable protein or DNA contaminants with agonistic TLR activity.
 
Activity:Specific activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at +4°C.
 
Handling:Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel.
 
581-011 012 013
Figure: TLR4-dependent proliferation of mouse splenocytesMethod: 4x105 mouse spleen cells were incubated in 0.2ml per well with 10µg/ml LPS or with medium only. Proliferation was determined in triplicates by 3H-thymidine incorporation.
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581-011 012 013

Product Literature References

Inhibition of SIK2 and SIK3 during differentiation enhances the anti-inflammatory phenotype of macrophages: N.J. Darling, et al.; Biochem. J. 474, 521 (2017), Abstract; Full Text
The Syk-NFAT-IL-2 Pathway in Dendritic Cells Is Required for Optimal Sterile Immunity Elicited by Alum Adjuvants: H.J. Khameneh, et al.; J. Immunol. 198, 196 (2017), Abstract; Full Text
Dynorphin 1-17 and Its N-Terminal Biotransformation Fragments Modulate Lipopolysaccharide-Stimulated Nuclear Factor-kappa B Nuclear Translocation, Interleukin-1beta and Tumor Necrosis Factor-alpha in Differentiated THP-1 Cells: S.S. Fazalul Rahiman, et al.; PLoS One 11, e0153005 (2016), Application(s): Cell culture, Abstract; Full Text
Lipopolysaccharide-induced multinuclear cells: increased internalization of polystyrene beads and possible signals for cell fusion: M. Nakanishi-Matsui, et al.; Biochem. Biophys. Res. Commun. 440, 611 (2013), Abstract;
Lipopolysaccharide induces multinuclear cell from RAW264. 7 line with increased phagocytosis activity: M. Nakanishi-Matsui, et al.; Biochem. Biophys. Res. Commun. 425, 144 (2012), Abstract;
Similarities and differences of innate immune responses elicited by smooth and rough LPS: I. Zanoni, et al.; Immunol. Lett. 142, 41 (2012), Abstract;
Stimulation of TLR4 by recombinant HSP70 requires structural integrity of the HSP70 protein itself: M. Luong, et al.; J. Inflamm (Lond) 9, 11 (2012), Abstract; Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;
CD14 controls the LPS-induced endocytosis of Toll-like receptor 4: I. Zanoni, et al.; Cell 147, 868 (2011), Abstract;
Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality: E. Marchlik, et al.; J. Leukoc. Biol. 88, 1171 (2010), Abstract;
R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006), Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry 9, 11 (1993), Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;

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