Hyaluronic Acid Fragments (<1,500 Da) (endotoxin-free) (Ready-to-Use)

TLR4 ligand



ALX-580-004-C250	250 µg	304.00 USD

Candidate for endogenous danger signal molecule for TLR4 (Toll-like receptor 4).

Product Specification

Alternative Name:	sHA (<1,500 Da) (endotoxin-free) (Ready-to-Use), Hyaluronan Oligosaccharide (<1,500 Da) (endotoxin-free) (Ready-to-Use)

Concentration:	1mg/ml

Purity Detail:	Absence of detectable protein or DNA contaminants with agonistic TLR activity. Endotoxin tested. The purity and size range of HA oligomer mixture has been confirmed by HPLC analysis and mass spectrometry.

Endotoxin Content:	<0.0002EU/µg

Purity:	≥95% (HPLC)

Formulation:	Liquid. Sterile, ready-to-use solution in PBS, pH 7.2.

Source/Host:	Isolated from bacteria.

Long Term Storage:	+4°C

Handling:	After opening, prepare aliquots and store at -20°C. Protect from light.

Miscellaneous/General:	Hyaluronic acid (HA) is a linear polysaccharide built from regularly alternating units of glucuronic acid and N-acetylglucosamine. Hyaluronan oligosaccharides are produced by partial digestion of high-molar mass hyaluronan with hyaluronidase followed by chromatographic fractionation into uniformly sized HA oligosaccharides by anion-exchange chromatography with the hyaluronidase removed.

Background / Technical Information:	Recommended working concentration for activation of TLR4-positive cells in vitro: 10-25µg/ml.

General Literature References

Oligosaccharides of Hyaluronan activate dendritic cells via toll-like receptor 4: C. Termeer, et al.; J. Exp. Med 195, 99 (2002), Abstract; Full Text