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Netrin-1 (human):Fc (human), (recombinant)

 
ALX-522-455-C010 10 µg 515.00 USD
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Product Specification

MW:~90kDa (SDS-PAGE)
 
Source:Produced in HEK 293 cells. The Fc portion of human IgG1 is fused to the C-terminus of the human netrin-1 (aa 25-604).
 
UniProt ID:O95631
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. From PBS containing trehalose and BRIJ 35.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Lonza).
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Binds to human, mouse and rat UNC5B.
 
Biological Activity:Induces axon outgrowth.
 
Reconstitution:Reconstitute with 100µl sterile distilled water. PBS containing at least 0.1% BSA should be used for further dilutions.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
522-455 01
Figure: Netrin-1 (human):Fc (human) (rec.) (Prod. No. ALX-522-455) induces outgrowth of the commisural axon.
Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody.
Picture courtesy of Dr Véronique Corset, Centre Léon Bérard, Lyon
522-455 2
Figure 2: Netrin-1 (human):Fc (human) (rec.) (Prod. No. AG-40B-0075) triggers a DCC-dependent phosphorylation of ERK1/2.
Method: HEK 293 cells (Cont.) or HEK 293 expressing the Netrin-1 receptor DCC (DCC) were incubated with Netrin-1 (h):Fc (h) (5nM) for the indicated time points (shown in minutes). Antibodies againts DCC, total ERK1/2 or phospho ERK1/2 were used, and visualized with a chemiluminescence detection system.
Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Léon Bérard, Lyon, France.
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522-455 01 522-455 2

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