Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Collagen I, rat tail (thin plate coating)

ALX-522-440-0050 50 ml 83.00 USD
Do you need bulk/larger quantities?

Product Specification

Alternative Name:COL1
MW:235kDa, 215kDa, 130kDa, 115kDa
Source:Isolated from rat tail tendons.
Formulation:Sterile liquid. In 60% ethanol.
Purity:≥90% (SDS-PAGE)
Endotoxin Content:<100 EU/mg purified protein (LAL test)
Appearance:Clear liquid.
Activity: Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips.
Application Notes:Can be used for thin layer coating of surfaces (culture  plates, slides, coverslips)
Shipping:Blue Ice Not Frozen
Short Term Storage:+4°C
Long Term Storage:+4°C
Handling:Do not expose to flames. Keep sterile. Do not freeze.
Scientific Background:Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.
Protocol:Thin coating procedure
  1. Ready to use formulation.
  2. Coat at 100µl/cm2.
  3. Place the collagen coated surface uncovered in a tissue culture hood overnight or until completely dry.
  4. Store at +4°C until use.
ALX-200-440 CHO
Figure 1. Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.
ALX-200-440 SDS-PAGE
Figure 2. Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, Rat Tail.
Please mouse over
ALX-200-440 CHO ALX-200-440 SDS-PAGE

Product Literature References

A rapid and efficient method for dissociated cultures of mouse myenteric neurons: G. Wahba, et al.; J. Neurosci. Methods 261, 110 (2016), Abstract;

General Literature References

Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tube: M.B. Bornstein, et al.; Lab. Invest. 134, (1958),

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Collagen Native protein
By catalog section:


Technical Service
Customer Service

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,