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Collagen I, rat tail (thin plate coating)

 
ALX-522-440-0050 50 ml 75.00 USD
 

Product Specification

Alternative Name:COL1
 
MW:235kDa, 215kDa, 130kDa, 115kDa
 
Source:Isolated from rat tail tendons.
 
Concentration:100µg/ml
 
Formulation:Sterile liquid. In 60% ethanol, 0.02N acetic acid.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<100 EU/mg purified protein (LAL test)
 
Appearance:Clear liquid.
 
Activity: Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips.
 
Application:Thin layer coating of surfaces (culture  plates, slides, coverslips)
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Handling:Do not expose to flames. Keep sterile. Do not freeze.
 
Miscellaneous/General:Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.
 
Protocol:Thin coating procedure
  1. Ready to use formulation.
  2. Coat at 100µl/cm2.
  3. Place the collagen coated surface uncovered in a tissue culture hood overnight or until completely dry.
  4. Store at +4°C until use.
 
ALX-200-440 CHO
Figure 1. Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.
ALX-200-440 SDS-PAGE
Figure 2. Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, Rat Tail.
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ALX-200-440 CHO ALX-200-440 SDS-PAGE

General Literature References

Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tube: M.B. Bornstein, et al.; Lab. Invest. 134, (1958),

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