United States
Collagen I, rat tail (thin plate coating)
| ALX-522-440-0050 | 50 ml | 75.00 USD |  |
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Product Specification
| Alternative Name: | COL1 |
| MW: | 235kDa, 215kDa, 130kDa, 115kDa |
| Source: | Isolated from rat tail tendons. |
| Concentration: | 100µg/ml |
| Formulation: | Sterile liquid. In 60% ethanol, 0.02N acetic acid. |
| Purity: | ≥90% (SDS-PAGE) |
| Endotoxin Content: | <100 EU/mg purified protein (LAL test) |
| Appearance: | Clear liquid. |
| Activity: | Tested in cell proliferation assay – Increased attachment of cells on collagen coated coverslips. |
| Hazard: | FLAMMABLE |
| Application: | Thin layer coating of surfaces (culture plates, slides, coverslips) |
| Short Term Storage: | +4°C |
| Long Term Storage: | +4°C |
| Handling: | Do not freeze. Keep sterile. Do not expose to flames. |
| Miscellaneous/General: | Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment. |
| Protocol: | Thin coating procedure
|
Figure 1. Glass coverslips were coated with or without collagen in 60% ethanol and allowed to dry overnight. CHO cells were plated at onto the coverslips and grown for two days at 37oC with 5% CO2. Cells were fixed in 10% buffered formalin. Coverslips were washed in PBS, mounted upside down onto microscope slides, and imaged with a 20X objective.
Figure 2. Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Collagen I, Rat Tail.
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General Literature References
Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tube: M.B. Bornstein, et al.; Lab. Invest. 134, (1958),
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