IL-12 (human):Fc (human), (recombinant)



ALX-522-146-C010	10 µg	315.00 USD

Product Specification

Alternative Name:	Interleukin-12, IL-12B:IL12A, IL-12 subunit p40:IL-12 subunit p35, CLMF p40:CLMF p35

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Lonza).

MW:	~100kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Induces proliferation of peripheral blood mononuclear cells (PBMC).

Source/Host:	Produced in HEK 293 cells. Human IL-12 subunit p40 (aa 23-328) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Specificity:	Binds to human IL-12R-β2.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Interleukin-12 (IL-12) belongs to the IL-12 cytokine family, which includes IL-12, IL-23, IL-27 and the member IL-35 [1]. IL-12, a disulfide-linked 70 kDa protein composed of the subunits p40 and p30, is primarily produced by antigen-presenting cells (APCs), such as macrophages, monocytes, and dendritic cells (DCs), following their activation by recognition of pathogen-specific patterns [2]. IL-12 induces secretion of IFN-γ and promotes T-helper 1 (Th1) cell proliferation and differentiation [2].

Background / Technical Information:	UniProt IDs:P29460: IL-12B (human)P29459: IL-12A (human)FLAG is a registered trademark of Sigma-Aldrich Co.

General Literature References

Interleukin-12 family members and the balance between rejection and tolerance: S. Goriely & M. Goldman; Curr. Opin. Organ Transplant. 13, 4 (2008), Abstract;

Interleukin-35: odd one out or part of the family?: L.W. Collison & D.A. Vignali; Immunol. Rev. 226, 248 (2008), Abstract;