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IL-12 (human):Fc (human), (recombinant)

 
ALX-522-146-C010 10 µg 361.00 USD
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Product Specification

Alternative Name:Interleukin-12, IL-12B:IL12A, IL-12 subunit p40:IL-12 subunit p35, CLMF p40:CLMF p35
 
MW:~100kDa
 
Source:Produced in HEK 293 cells. Human IL-12 subunit p40 (aa 23-328) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG®-tag.
 
UniProt ID:P29459 (IL-12A), P29460 (IL-12B)
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Lonza).
 
Specificity:Binds to human IL-12R-β2.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Interleukin-12 (IL-12) belongs to the IL-12 cytokine family, which includes IL-12, IL-23, IL-27 and the member IL-35 [1]. IL-12, a disulfide-linked 70 kDa protein composed of the subunits p40 and p30, is primarily produced by antigen-presenting cells (APCs), such as macrophages, monocytes, and dendritic cells (DCs), following their activation by recognition of pathogen-specific patterns. IL-12 induces secretion of IFN-γ and promotes T-helper 1 (Th1) cell proliferation and differentiation. Historical results have shown that IL-12 induces proliferation of peripheral blood mononuclear cells (PBMC).
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 

General Literature References

Interleukin-12 family members and the balance between rejection and tolerance: S. Goriely & M. Goldman; Curr. Opin. Organ Transplant. 13, 4 (2008), Abstract;
Interleukin-35: odd one out or part of the family?: L.W. Collison & D.A. Vignali; Immunol. Rev. 226, 248 (2008), Abstract;

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