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IL-35 (human):Fc (human), (recombinant)

Highly pure interleukin involved in T cell regulation.
 
ALX-522-140-C010 10 µg 401.00 USD
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Product Specification

Alternative Name:Interleukin-35, EBI3:IL-12A, IL-27B:CLMF p35
 
MW:~85kDa
 
Source:Produced in HEK 293 cells. Human EBI3 (aa 21-229) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG®-tag.
 
UniProt ID: Q14213 (IL-27B), P29459 (IL-21A)
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:EBI3 (Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL-27B; IL-27 subunit β) and IL-12A (IL-12 subunit p35; CLMF p35) form the heterodimeric cytokine IL-35 (interleukin-35) involved in T cell regulation. IL-35 is highly expressed in regulatory T cell (Treg) but not in effector or naive T cells. IL-35 is required for the suppressive function of T-reg in vitro and in vivo.
 
Technical Info/Product Notes:Historical data has shown that IL-35 binds to IL-12Rß2, and it has been demonstrated in the literature to inhibit mononuclear cell proliferation.

FLAG is a registered trademark of Sigma-Aldrich Co.
 

Product Literature References

IL-35 Inhibits Angiogenesis through VEGF/Ang2/Tie2 Pathway in Rheumatoid Arthritis: S. Jiang, et al.; Cell. Physiol. Biochem. 40, 1105 (2016), Abstract;
Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway: X. Sha, et al.; J. Biol. Chem. 290, 19307 (2015), Application(s): Cell culture, Abstract;
Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis: M. Filková, et al.; Cytokine 73, 36 (2015), Application(s): Cell Culture: IHC, IF used to analyze IL-35 in PBMCs, Abstract;
Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway: Q. Chen, et al.; PLoS One 8, e59194 (2013), Application(s): Treatment of monocytes-derived dendritic cells, Abstract; Full Text
Regulatory T-Cell (Treg) hybridoma as a novel tool to study Foxp3 regulation and Treg fate: R. Sharma, et al.; J. Autoimmun. 37, 113 (2011), Abstract;
Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC: M. Seyerl, et al.; Eur. J. Immunol. 40, 321 (2010), Abstract;

General Literature References

IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells: W. Niedbala, et al.; Eur. J. Immunol. 37, 3021 (2007), Abstract; Full Text
The inhibitory cytokine IL-35 contributes to regulatory T-cell function: L.W. Collison, et al. ; Nature 450, 566 (2007), Abstract;
Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin: O. Devergne, et al.; PNAS 94, 12041 (1997), Abstract; Full Text

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