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TNC:TWEAK (soluble) (mouse), (recombinant)

 
ALX-522-138-C010 10 µg 370.00 USD
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Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor. Note: For some TNF ligands, such as FasL (mouse), activated by further oligomerization (> hexamer), the presence of TNC is not sufficient and ligand crosslinking using the "Enhancer for Ligands" (Prod. No. ALX-804-034) is required.

Product Specification

Alternative Name:Apo-3L, TNFSF 12
 
MW:~30kDa
 
Source:Produced in HEK 293 cells. The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG®-tag.
 
UniProt ID:O54907
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Specificity:Binds to Fn14 (mouse):Fc (human) (rec.) (Prod. No. ALX-522-036)
 
Applications:ELISA
 
Application Notes:ELISA: binds to mouse Fn14.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 

General Literature References

Enforced covalent trimerization increases the activity of the TNF ligand family members TRAIL and CD95L: D. Berg, et al.; Cell Death Differ. 14, 2021 (2007), Abstract;

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Fn14 (mouse):Fc (human), (recombinant) 

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Enhancer for Ligands 

Enhances biological activity of TRAIL, soluble (human) (recombinant) and FasL, soluble (human) (recombinant).
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ALX-804-034-C050 50 µg 260.00 USD
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