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Nesfatin-1 (rat), (recombinant)

 
ALX-522-116-C010 10 µg 391.00 USD
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Product Specification

Alternative Name:Gastric cancer antigen Zg4, N-terminal fragment of NUCB2, NEFA/nucleobindin2-encoded satiety- and fat-influencing protein
 
MW:~8kDa
 
Source:Recombinant rat nesfatin-1 (aa 25-106) produced in E. coli.
 
UniProt ID:Q9JI85
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 0.2% bovine serum albumin (BSA).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
 

General Literature References

Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006), Abstract;

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