Netrin-4 (human), (recombinant)



ALX-522-113-2010	2x10 µg	342.00 USD

Product Specification

Alternative Name:	β-Netrin, Hepar-derived netrin-like protein

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~70kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS and 5% trehalose.

Source/Host:	Produced in HEK 293 cells. Human netrin-4 is fused at the N-terminus to a FLAG^®-tag.

Reconstitution:	Reconstitute each vial with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The netrins (whose name means "one who guides" in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, netrin-4/b and G-netrins have been cloned. The netrins are involved in axonal guidance, are required for regeneration and maintenance of the central nervous system and have been implicated in the development of mammary gland, lung, pancreas, and blood vessel. Netrin-4 acts during the development and control the migration of neurons, being chemoattractive for some neurons and chemorepellent for others. Netrin-4 is broadly expressed in both neural and non-neural tissues. The role of netrin-4 in guiding neurones is mediated by known netrin-1 receptors: DCC and UNC5H1. Netrin-4 promotes angiogenesis by stimulating proliferation and migration of microvascular endothelial cells to the same extent that vascular endothelial growth factor (VEGF).

Background / Technical Information:	UniProt ID Q9HB63: Netrin-4 (human)FLAG is a registered trademark of Sigma-Aldrich Co.