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DCC (fibronectin domain 5) (human), (recombinant) (GST-tag)

 
ALX-522-109-C050 50 µg 425.00 USD
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Product Specification

Alternative Name:Deleted in colorectal cancer protein
 
Source:Produced in E. coli. The fibronectin domain 5 (FBN5) of human DCC (aa 841-941) is fused to an N-terminal GST-tag.
 
UniProt ID:P43146
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse netrin-1.
 
Applications:ELISA
 
Application Notes:ELISA: binds to netrin-1 at 500-4000 ng/ml.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 

Product Literature References

Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer: J. Fitamant, et al.; PNAS 105, 4850 (2008), Abstract;

General Literature References

Netrin binds discrete subdomains of DCC and UNC5 and mediates interactions between DCC and heparin: B.V. Geisbrecht, et al.; J. Biol. Chem. 278, 32561 (2003), Abstract;

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