Product Details
| Alternative Name: | Repulsive guidance molecule A, RGM domain family member A |
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| Source: | Produced in HEK 293 cells. Human RGMA (aa 48-422) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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| UniProt ID: | Q96B86 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.) |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | The RGMA is an GPI-anchored protein involved in the axon guidance and is predominantly expressed in the developing and adult central nervous system in mouse. RGMA inhibits mammalian CNS neurite outgrowth. The protein acts as co-receptor of the bone morphogenetic protein. The mature RGMA protein is proteolytically processed and is glycosylated. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The Neogenin Intracellular Domain Regulates Gene Transcription via Nuclear Translocation: D. Goldschneider, et al.; Mol. Cell. Biol.
28, 4068 (2008),
Abstract;
General Literature References
RGMa inhibition promotes axonal growth and recovery after spinal cord injury: K. Hata, et al.; J. Cell Biol.
173, 47 (2006),
Abstract;
Repulsive guidance molecule (RGMa), a DRAGON homologue, is a bone morphogenetic protein co-receptor: J.L. Babitt, et al.; J. Biol. Chem.
280, 29820 (2005),
Abstract;
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