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Netrin-1 (human), (recombinant)

ALX-522-100-C010 10 µg 255.00 USD
ALX-522-100-2010 2x10 µg 458.00 USD
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Product Specification

Source:Produced in HEK 293 cells. The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a FLAG®-tag.
UniProt ID:O95631
Concentration:0.1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥90% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Species reactivity:Human, Mouse, Rat
Specificity:Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC.
Application Notes:ELISA: binds to UNC5B at 15-1000 ng/ml.
Reconstitution:Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Historical results have shown that netrin-1 induces axon outgrowth and inhibits DCC- and UNC5H2-mediated apoptosis in HEK 293 cells.
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
Figure: Netrin-1 (human) (rec.) (Prod. No. ALX-522-100) mediated commissural axon outgrowth. Method: Dorsal spinal cord were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of Netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Véronique Corset, Centre Léon Bérard, Lyon.
ALX-522-100 SDS-PAGE
SDS-PAGE analysis of Netrin-1 (human), (recombinant): Lane 1: MW Marker, Lane 2: 1μg Netrin-1.
ALX-522-100 ELISA
ELISA analysis of Netrin-1 (human), (recombinant): Each well is coated with either 500ng Unc5b (Prod. No. ALX-522-095) or 2% BSA. Netrin-1 is titrated in 2-fold series and detected by anti-FLAG antibody.
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522-100 ALX-522-100 SDS-PAGE ALX-522-100 ELISA

Product Literature References

CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development: T. Tu, et al.; Cell Res. 25, 275 (2015), Application(s): Immunoprecipitation, Abstract; Full Text
The bipartite Rac1 guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (Elmo1/Dock180) protects endothelial cells from apoptosis in blood vessel development: K. Schaeker, et al.; J. Biol. Chem. 290, 6408 (2015), Application(s): Western Blotting, Abstract; Full Text
Interference With Netrin-1 and Tumor Cell Death in Non-Small Cell Lung Cancer: C. Delloye-Bourgeois, et al.; J. Natl. Cancer Inst. 101, 237 (2009), Abstract;
Netrin-1 acts as a survival factor for aggressive neuroblastoma: C. Delloye-Bourgeois, et al.; J. Exp. Med. 206, 833 (2009), Abstract;
Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production: F.C. Lourenço, et al.; Cell Death Differ. 16, 655 (2009), Abstract;
Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer: J. Fitamant, et al.; PNAS 105, 4850 (2008), Abstract;

General Literature References

Netrin-1: when a neuronal guidance cue turns out to be a regulator of tumorigenesis: P. Mehlen & C. Furne; Cell. Mol. Life Sci. 62, 2599 (2005), (Review), Abstract;
Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer 4, 978 (2004), (Review), Abstract;

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