Product Details
| MW: | ~85kDa |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a FLAG®-tag. |
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| UniProt ID: | O95631 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Species reactivity: | Human, Mouse, Rat
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| Specificity: | Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to UNC5B at 15-1000 ng/ml. |
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| Reconstitution: | Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Historical results have shown that netrin-1 induces axon outgrowth and inhibits DCC- and UNC5H2-mediated apoptosis in HEK 293 cells. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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ELISA analysis of Netrin-1 (human), (recombinant): Each well is coated with either 500ng Unc5b (Prod. No. ALX-522-095) or 2% BSA. Netrin-1 is titrated in 2-fold series and detected by anti-FLAG antibody.
SDS-PAGE analysis of Netrin-1 (human), (recombinant): Lane 1: MW Marker, Lane 2: 1μg Netrin-1.
Figure: Netrin-1 (human) (rec.) (Prod. No.
ALX-522-100) mediated commissural axon outgrowth.
Method: Dorsal spinal cord were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of Netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody.
Picture courtesy of Véronique Corset, Centre Léon Bérard, Lyon."
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Product Literature References
CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development: T. Tu, et al.; Cell Res.
25, 275 (2015),
Application(s): Immunoprecipitation,
Abstract;
Full Text
The bipartite Rac1 guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (Elmo1/Dock180) protects endothelial cells from apoptosis in blood vessel development: K. Schaeker, et al.; J. Biol. Chem.
290, 6408 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Interference With Netrin-1 and Tumor Cell Death in Non-Small Cell Lung Cancer: C. Delloye-Bourgeois, et al.; J. Natl. Cancer Inst.
101, 237 (2009),
Abstract;
Netrin-1 acts as a survival factor for aggressive neuroblastoma: C. Delloye-Bourgeois, et al.; J. Exp. Med.
206, 833 (2009),
Abstract;
Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production: F.C. Lourenço, et al.; Cell Death Differ.
16, 655 (2009),
Abstract;
Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer: J. Fitamant, et al.; PNAS
105, 4850 (2008),
Abstract;
General Literature References
Netrin-1: when a neuronal guidance cue turns out to be a regulator of tumorigenesis: P. Mehlen & C. Furne; Cell. Mol. Life Sci.
62, 2599 (2005), (Review),
Abstract;
Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer
4, 978 (2004), (Review),
Abstract;
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