LAG-3 (mouse):Fc (mouse) (recombinant)

ALX-522-099

Revised 01-Feb-10

LAG-3 (mouse):Fc (mouse) (recombinant)
SYNONYMS	Lymphocyte Activation Gene-3 (mouse):Fc (mouse) (recombinant) FDC Protein (mouse):Fc (mouse) (recombinant) CD223 (mouse):Fc (mouse) (recombinant)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Dendritic Cells [DC]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-099-C050		50 µg	434.00 USD
ALX-522-099-C500		500 µg	1'625.00 USD

Product Specification

MW:	~180kDa (SPS-PAGE, nonreducing), ~80kDa (SPS-PAGE, reducing)
SOURCE/HOST:	Produced in CHO cells. The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH₂-CH₃).
CONCENTRATION:	0.5mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS.
ENDOTOXIN CONTENT:	<1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds to human and mouse MHC class II.
BIOLOGICAL ACTIVITY:	Induces maturation of mouse dendritic cells in vitro. Induces CD4 and CD8 antigen-specific T cells responses when coinjected s.c. in mice, together with the antigen.
APPLICATION:	Flow Cytometry
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at +4°C.

Product Specific Literature References

LAG-3 enables DNA vaccination to persistently prevent mammary carcinogenesis in HER-2/neu transgenic BALB/c mice: P. Cappello, et al.; Cancer Res. 63, 2518 (2003) Abstract; Full Text

General Information

The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4 [1]. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa) [1,2]. The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome [3]. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses [4].
BACKGROUND/TECHNICAL INFORMATION
Swiss Prot link Q61790: Lymphocyte activation gene-3 protein (mouse) (Precursor)

General Literature References

[1] LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination: F. Triebel; Trends Immunol. 24, 619 (2003) Abstract
[2] A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006) Abstract
[3] Active tuberculosis in Africa is associated with reduced Th1 and increased Th2 activity in vivo: C. Lienhardt, et al.; Eur. J. Immunol. 32, 1605 (2002) Abstract
[4] Expression and release of LAG-3-encoded protein by human CD4+ T cells are associated with IFN-gamma production: F. Annunziato, et al.; FASEB J. 10, 769 (1996) Abstract

Further Categories Containing This Product:

CD Proteins • Recombinant Proteins / Fusion Proteins