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LAG-3 (mouse):Fc (mouse), (recombinant)

 
ALX-522-099-C050 50 µg 498.00 USD
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Product Specification

Alternative Name:Lymphocyte activation gene-3, FDC protein, CD223
 
MW:~180kDa (SPS-PAGE, nonreducing), ~80kDa (SPS-PAGE, reducing)
 
Source:Produced in CHO cells. The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH2-CH3).
 
UniProt ID:Q61790
 
Concentration:0.5mg/ml
 
Formulation:Liquid. In PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse MHC class II.
 
Biological Activity:Induces maturation of mouse dendritic cells in vitro. Induces CD4 and CD8 antigen-specific T cells responses when coinjected s.c. in mice, together with the antigen.
 
Applications:Flow Cytometry
 
Reconstitution:Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Scientific Background:The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
 

Product Literature References

LAG-3 enables DNA vaccination to persistently prevent mammary carcinogenesis in HER-2/neu transgenic BALB/c mice: P. Cappello, et al.; Cancer Res. 63, 2518 (2003), Abstract; Full Text

General Literature References

A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006), Abstract;
LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination: F. Triebel; Trends Immunol. 24, 619 (2003), Abstract;
Active tuberculosis in Africa is associated with reduced Th1 and increased Th2 activity in vivo: C. Lienhardt, et al.; Eur. J. Immunol. 32, 1605 (2002), Abstract;
Expression and release of LAG-3-encoded protein by human CD4+ T cells are associated with IFN-gamma production: F. Annunziato, et al.; FASEB J. 10, 769 (1996), Abstract;

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