Adiponectin (globular domain) (mouse), (recombinant)



ALX-522-088-C050	50 µg	350.00 USD

Product Specification

Alternative Name:	ACRP30, Adipocyte complement related protein of 30kDa, AdipoQ, apM1, GBP28

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg protein (LAL test; BioWhittaker).

MW:	~26kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Source/Host:	Produced in HEK 293 cells. The globular domain of mouse adiponectin (aa 111-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Adiponectin, also known as ACRP30 (30kDa adipocyte complement-related protein) is a hormone synthesized and secreted by adipocytes. Expression and plasma levels of adiponectin are decreased in insulin resistance and obesity and synthesis of this adipokine increases when insulin sensitivity is improved and weight is lost. Adiponectin consists of a carboxyl-terminal globular domain and an amino-terminal collagen-like domain containing 22 Gly-X-Y repeats. The presence of a small amount of a carboxyl-terminal fragment containing the globular domain of adiponectin (gAdiponectin) has been demonstrated in human serum by immunoprecipitation. Adiponectin and gAdiponectin act in the brain to increase energy expenditure and to decrease body weight.

Background / Technical Information:	FLAG is a registered trademark of Sigma-Aldrich Co.

General Literature References

Adiponectin acts in the brain to decrease body weight: Y. Qi, et al.; Nat. Med. 10, 524 (2004), Abstract;

Adiponectin, obesity, and cardiovascular disease: M. Fasshauer, et al.; Biochimie 86, 779 (2004), Abstract;

Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice: J. Fruebis, et al.; PNAS 98, 2005 (2001), Abstract; Full Text