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Fc (human):TNF-α (soluble) (human), (recombinant)

 
ALX-522-087-C010 10 µg 418.00 USD
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Product Specification

Alternative Name:Tumor necrosis factor α, TNFSF 2
 
MW:~48kDa (calculated), ~52kDa (observed)
 
Source:Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
 
UniProt ID:P01375
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that TNFα induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer).
 
ALX-522-087 SDS-PAGE
SDS-PAGE analysis of Fc (human):TNF-α (soluble) (human), (recombinant): Lane 1: MW Marker, Lane 2: 1µg TNF-α.
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ALX-522-087 SDS-PAGE

General Literature References

Life and death decisions: secondary complexes and lipid rafts in TNF receptor family signal transduction: J.R. Muppidi, et al.; Immunity 21, 461 (2004), Abstract;

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