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Fn14 (human):Fc (human), (recombinant)

 
ALX-522-086-C050 50 µg 419.00 USD
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Product Specification

Alternative Name:Fibroblast growth factor-inducible immediate-early response protein 14, TNFRSF 12A, TWEAK-R, CD266
 
MW:~36kDa for the chimeric protein (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human Fn14 (aa 1-75) is fused at the C-terminus to the Fc portion of human IgG1 and a linker peptide (2 aa).
 
UniProt ID:Q9NP84
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse TWEAK.
 
Applications:ELISA
 
Application Notes:ELISA: binds to TWEAK at 1-10 ng/ml.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least six months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:The fibroblast growth factor-inducible-14 (Fn14) is a 129 aa type Ia transmembrane protein. Fn14 is a growth-factor regulated, immediate-early response protein. It is expressed in a developmental and adult tissue-specific manner. Fn14 expression decreases cellular adhesion to extracellular matrix proteins fibronectin and vitronectin and reduces cell growth and migration. It is expressed at high levels in liver cancer cells, in hepatocellular carcinoma specimens as well as in regenerating liver cells showing Fn14 may play a role in hepatocyte growth and liver neoplasia. Fn14 binds to TWEAK (Apo-3 Ligand), a member of the TNF family. Historical data has shown that Fn14 inhibits TWEAK-mediated killing of Kym-1 target cells.
 

Product Literature References

Intraovarian TWEAK/Fn14 ligand-receptor system limits ovarian preovulatory follicles from excessive luteinization: A. De, et al.; Mol. Endocrinol. 20, 2528 (2006), Abstract; Full Text

General Literature References

The molecular architecture of the TNF superfamily: J.L. Bodmer, et al.; TIBS 27, 19 (2002), Abstract;
A novel TNF receptor family member binds TWEAK and is implicated in angiogenesis: S.R. Wiley, et al.; Immunity 15, 837 (2001), Abstract;

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