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Fc (human):CD40L (soluble) (human), (recombinant)

 
ALX-522-076-C010 10 µg 425.00 USD
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Product Specification

Alternative Name:CD154, TNFSF 5, gp39
 
MW:~58kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
 
UniProt ID:P29965
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; BioWhittaker).
 
Specificity:Binds to human CD40.
 
Applications:ELISA, FUNC
 
Application Notes:ELISA: binds to human CD40:COMP at 2-50 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that Fc (human):CD40L stimulates the proliferation of human dendritic cells. Note: Does not require an enhancer.
 
522-076
Figure: Proliferation of human dendritic cells (DC) induced by Fc (human):CD40L, Soluble (human) (rec.) (Prod. No. ALX-522-076).Method: Human DC were derived from GMCSF/IL-4 treated monocytes. The so obtained DC, 105 cells/assay, were incubated with 3µg/ml Fc (human):CD40L, Soluble (human) (rec.) (A), 3µg/ml boiled Fc (human):CD40L, Soluble (human) (rec.) (B), 3µg/ml control:Fc (C) and media (D). After 18 hours, DC maturation was analyzed by immunostaining with a CD83-specific MAb and cytofluorimetry analysis. Indicated are the percentages of activated DC.
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