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CD40L (soluble) (mouse), (recombinant)

 
ALX-522-070-C010 10 µg 240.00 USD
 
ALX-522-070-2010 2x10 µg 431.00 USD
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Product Specification

Alternative Name:CD154, TNFSF 5, gp39
 
MW:~26kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
UniProt ID:P27548
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<50EU/mg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse CD40.
 
Applications:ELISA
 
Application Notes:ELISA: binds to human CD40 at 10-500 ng/ml.
 
Reconstitution:Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that mouse CD40L stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1'000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer (see Set Prod. No. ALX-850-075).

FLAG is a registered trademark of Sigma-Aldrich Co.
 

Product Literature References

IgE and IgA produced by OX40-OX40L or CD40-CD40L interaction in B cells-mast cells re-activate FcεRI or FcαRI on mast cells in mouse allergic asthma: G. Ui Hong, et al.; Eur. J. Pharmacol. 754, 199 (2015), Application(s): Cell Culture, Abstract;

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