Adiponectin (human), (recombinant)



ALX-522-063-C050	50 µg	335.00 USD

Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species.

Product Specification

Alternative Name:	Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~33kDa (SDS-PAGE).

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Activates AMPK.

Source/Host:	Produced in HEK 293 cells. Human adiponectin (aa 16-244) is fused at the N-terminus to a linker peptide (14 aa) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q15848: Adiponectin (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure: Superdex 200 analysis of Adiponectin (human) (recombinant) (Prod. No. ALX-522-063) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin. For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References.

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Product Literature References

Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase: A. Suzuki, et al.; Mol. Cell. Biol. 27, 4317 (2007), Abstract;

General Literature References

Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004), Abstract; Full Text

ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism: A.H. Berg, et al.; Trends Endocrinol. Metab. 13, 84 (2002), (Review), Abstract;

Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002), Abstract; Full Text