GITR (human):Fc (human), (recombinant)



ALX-522-061-C050	50 µg	355.00 USD

Product Specification

Alternative Name:	AITR, TNFRSF 18, Glucocorticoid-induced TNFR-related protein, Activation-inducible TNFR family receptor

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~47kDa (SDS-PAGE).

Quantity:	50µg

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Source/Host:	Produced in HEK 293 cells. The cysteine-rich region of human GITR (AITR) (aa 26-161) is fused to the Fc portion of human IgG1.

Reconstitution:	Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Specificity:	Binds human GITRL (AITRL).

Short Term Storage:	-20°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored as recommended.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q9Y5U5: GITR (human)

Figure: Receptor binding assay: a 96 well ELISA plate was coated O/N with 50ng GITR:Fc per well. After a blocking step, 100µl of supernatant containing transiently expressed hGITRL (diamonds) or hFasL (squares), were added to the first well. To each well were subsequently added 100µl of the previous one in a 1:1 dilution . After extensive washing, enhancer for ligands (Prod. No. ALX-804-034) at 1µg/ml was added for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP (1:1'000 dilution) incubated for 1 hour. OPD was used as a substrate, absorbance was measured at 490 nm in an ELISA reader.

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Product Literature References

Identification of glucocorticoid-induced TNF receptor-related protein ligand on keratinocytes: ligation by GITR induces keratinocyte chemokine production and augments T-cell proliferation: A.M. Byrne, et al.; J. Invest. Dermatol. 129, 2784 (2009), Abstract; Full Text