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BAFF-R (human):Fc (human), (recombinant)

 
ALX-522-060-C050 50 µg 372.00 USD
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Product Specification

Alternative Name:TNFRSF 13C, BR3, BlySR3, CD268
 
MW:~40kDa (SDS-PAGE)
 
Source:Produced in HEK 293 cells. The extracellular domain of human BAFF-R (aa 2-71) is fused at the C-terminus to the Fc portion of human IgG1.
 
UniProt ID:Q96RJ3
 
Concentration:1mg/ml (after reconstitution)
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse BAFF.
 
Applications:ELISA
 
Application Notes:ELISA: binds to BAFF.
Detection of membrane-bound human and mouse BAFF in combination with PAb to human IgG1.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical results have shown that BAFF-R inhibits rhsBAFF mediated splenocyte survival.
 
522-060
Figure: rhBAFF-R:Fc inhibits rhsBAFF-mediated activation of freshly isolated mouse splenocytes. Concentration of rhBAFF-R:Fc required to achieve complete inhibition depends on the concentration of rhsBAFF (Prod. No. ALX-522-025) used. A ratio (rhsBAFF:rhBAFF-R:Fc) 1:50 and higher is recommended.Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed on FACS, and the B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media only, with the indicated concentration of rhsBAFF (Prod. No. ALX-522-025) alone or in the presence of excess rhsBAFF-R:Fc (+5µg/ml). After 3 days in culture, cells were again analyzed on FACS with the saved settings. Ratio of % living/% dead cells was calculated and plotted.
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522-060

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