Adiponectin (mouse), (recombinant)



ALX-522-059-C050	50 µg	320.00 USD
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Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species.

Product Specification

Alternative Name:	Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28

Concentration:	0.5mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~35kDa (SDS-PAGE).

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Inhibits serum glucose production in mice. Protects mouse hepatocytes from TNF-α-induced cell death in vitro.

Source/Host:	Produced in HEK 293 cells. Mouse adiponectin (aa 18-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q60994: Adiponectin (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: Bioactivity of rmsAdiponectin.Method: 2 groups of 4 mice each were injected with PBS (vehicle), rmAdiponectin (Prod. No. ALX-522-059) and rhACRP30headless (tail adiponectin) (Prod. No. ALX-203-002) at 1µg/g body weight as a tail vein injection. Subsequently, serum glucose levels were monitored over the time indicated by Trinder assay. Plotted is the average of 4 mice. rmAdiponectin represses hepatic glucose production, so serum glucose levels decrease until counter-regulatory mechanisms (i.e. glucagon) become effective. rhACRP30headless demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in this in vivo assay.

Figure 2: Superdex 200 analysis of Adiponectin (mouse) (recombinant) (Prod. No. ALX-522-059) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin. For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References.

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Product Literature References

Regulation of T-cell mediated hepatic inflammation by adiponectin and leptin: J.A. Sennello, et al.; Endocrinology 146, 2157 (2005), Abstract;

General Literature References

Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004), Abstract; Full Text

Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002), Abstract; Full Text