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Adiponectin (mouse), (recombinant)

 
ALX-522-059-C050 50 µg 349.00 USD
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  • Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species
  • Expressed in mammalian cell line to guarantee proper post-translational modifications
  • High purity and low endotoxin content to ensure experimental success

Product Specification

Alternative Name:Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28
 
MW:~35kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. Mouse adiponectin (aa 18-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
UniProt ID:Q60994
 
Concentration:0.5mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Reconstitution:Reconstitute with 100µl sterile water.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that adiponection inhibits serum glucose production in mice and protects mouse hepatocytes from TNF-α-induced cell death in vitro.

FLAG is a registered trademark of Sigma-Aldrich Co.
 
203-002
Figure 1: Bioactivity of rmsAdiponectin.Method: 2 groups of 4 mice each were injected with PBS (vehicle), rmAdiponectin (Prod. No. ALX-522-059) and rhACRP30headless (tail adiponectin) (Prod. No. ALX-203-002) at 1µg/g body weight as a tail vein injection. Subsequently, serum glucose levels were monitored over the time indicated by Trinder assay. Plotted is the average of 4 mice. rmAdiponectin represses hepatic glucose production, so serum glucose levels decrease until counter-regulatory mechanisms (i.e. glucagon) become effective. rhACRP30headless demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in this in vivo assay.
522-059 2
Figure 2: Superdex 200 analysis of Adiponectin (mouse) (recombinant) (Prod. No. ALX-522-059) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin. For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References.
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203-002 522-059 2

Product Literature References

Chronic maternal infusion of full-length adiponectin in pregnant mice down-regulates placental amino acid transporter activity and expression and decreases fetal growth: F.J. Rosario, et al.; J. Physiol. 590, 1495 (2012), Abstract; Full Text
Regulation of T-cell mediated hepatic inflammation by adiponectin and leptin: J.A. Sennello, et al.; Endocrinology 146, 2157 (2005), Abstract;

General Literature References

Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004), Abstract; Full Text
Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002), Abstract; Full Text

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