- Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species
- Expressed in mammalian cell line to guarantee proper post-translational modifications
- High purity and low endotoxin content to ensure experimental success
Product Details
| Alternative Name: | Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28 |
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| MW: | ~35kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. Mouse adiponectin (aa 18-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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| UniProt ID: | Q60994 |
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| Concentration: | 0.5mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Reconstitution: | Reconstitute with 100µl sterile water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that adiponection inhibits serum glucose production in mice and protects mouse hepatocytes from TNF-α-induced cell death in vitro.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Bioactivity of rmsAdiponectin.
Method: 2 groups of 4 mice each were injected with PBS (vehicle), rmAdiponectin (Prod. No.
ALX-522-059) and rhACRP30
headless (tail adiponectin) (Prod. No.
ALX-203-002) at 1µg/g body weight as a tail vein injection. Subsequently, serum glucose levels were monitored over the time indicated by Trinder assay. Plotted is the average of 4 mice. rmAdiponectin represses hepatic glucose production, so serum glucose levels decrease until counter-regulatory mechanisms (i.e. glucagon) become effective. rhACRP30
headless demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in this
in vivo assay."
Figure 2: Superdex 200 analysis of Adiponectin (mouse) (recombinant) (Prod. No.
ALX-522-059) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin.
For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References."
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Product Literature References
Adiponectin ameliorates placental injury in gestational diabetes mice by correcting fatty acid oxidation/peroxide imbalance-induced ferroptosis via restoration of CPT-1 activity: Y. Zheng, et al.; Endocrine
75, 781 (2022),
Application(s): In vivo use,
Abstract;
Adiponectin regulates electroacupuncture-produced analgesic effects in association with a crosstalk between the peripheral circulation and the spinal cord: Z. Ning, et al.; Brain Behav. Immun.
99, 43 (2022),
Application(s): In vivo use,
Abstract;
AdipoRon attenuates Wnt signaling by reducing cholesterol-dependent plasma membrane rigidity: M.L. Salinas, et al.; Biophys. J.
118, 885 (2020),
Application(s): In vitro use with cells,
Abstract;
Full Text
Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor: D. Zhang, et al.; Cell Commun. Signal.
18, 60 (2020),
Application(s): In vitro use with cells,
Abstract;
Full Text
Normalisation of circulating adiponectin levels in obese pregnant mice prevents cardiac dysfunction in adult offspring: O.R. Vaughan, et al.; Int. J. Obes.
44, 488 (2020),
Application(s): In vivo use,
Abstract;
Full Text
Normalizing adiponectin levels in obese pregnant mice prevents adverse metabolic outcomes in offspring: M.E. Paulsen, et al.; FASEB J.
33, 2899 (2019),
Abstract;
Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease: Y.J. Peng, et al.; J. Biomed. Sci.
25, 24 (2018),
Abstract;
Full Text
Photoreceptor glucose metabolism determines normal retinal vascular growth: Z. Fu, et al.; EMBO Mol. Med.
10, 76 (2018),
Abstract;
Full Text
Adiponectin supplementation in pregnant mice prevents the adverse effects of maternal obesity on placental function and fetal growth: I.L. Aye, et al.; PNAS
112, 12858 (2015),
Abstract;
Full Text
Chronic maternal infusion of full-length adiponectin in pregnant mice down-regulates placental amino acid transporter activity and expression and decreases fetal growth: F.J. Rosario, et al.; J. Physiol.
590, 1495 (2012),
Abstract;
Full Text
Regulation of T-cell mediated hepatic inflammation by adiponectin and leptin: J.A. Sennello, et al.; Endocrinology
146, 2157 (2005),
Abstract;
General Literature References
Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem.
279, 12152 (2004),
Abstract;
Full Text
Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem.
277, 29359 (2002),
Abstract;
Full Text
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