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DcR3 (human):Fc (human), (recombinant)

 
ALX-522-055-C050 50 µg 406.00 USD
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Product Specification

Alternative Name:TNFRSF 6B, Decoy receptor 3
 
MW:~58kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human DcR3 (decoy receptor 3) (aa 1-246) is fused to the Fc portion of human IgG1.
 
UniProt ID:O95407
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Specificity:Binds human LIGHT. Does not bind human FasL.
 
Applications:ELISA
 
Application Notes:ELISA: binds to LIGHT.
Detection of human LIGHT and TL1A (VEGI) in combination with PAb to human IgG1.
 
Reconstitution:Reconstitute with 50µl sterile water.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
522-055
Figure:
Method: A 96-well ELISA plate was coated O/N with 50 ng rh DcR3:Fc  (Prod. No. ALX-522-055) per well. After blocking, the indicated concentrations of rhsFasL (Prod. No. ALX-522-001), rhsSuperFasL (Prod. No. ALX-522-020) or rhsLIGHT (Prod. No. ALX-522-018),  were added for 1 hour. After extensive washing, enhancer for ligands (Prod. No. ALX-804-034) at 1µg/ml was added and incubated for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP (1:1000 dilution, Jackson Lab.) incubated for 1 hour. OPD was used as a substrate, and absorbance was measured at 490 nm in an ELISA reader. 
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Product Literature References

A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis: K.Y. Yu, et al.; J. Biol. Chem. 274, 13733 (1999), Abstract; Full Text
Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer: R.M. Pitti, et al.; Nature 396, 699 (1998), Abstract;

General Literature References

Modulation of dendritic cell differentiation and maturation by decoy receptor 3: T.L. Hsu, et al.; J. Immunol. 168, 4846 (2002), Abstract;
Soluble decoy receptor 3 is expressed by malignant gliomas and suppresses CD95 ligand-induced apoptosis and chemotaxis: W. Roth, et al.; Cancer Res. 61, 2759 (2001), Abstract;

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