Product Details
| Alternative Name: | TNFRSF 6B, Decoy receptor 3 |
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| MW: | ~58kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human DcR3 (decoy receptor 3) (aa 1-246) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | O95407 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Specificity: | Binds human LIGHT. Does not bind human FasL. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to LIGHT.
Detection of human LIGHT and TL1A (VEGI) in combination with PAb to human IgG1. |
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| Reconstitution: | Reconstitute with 50µl sterile water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure:
Method: A 96-well ELISA plate was coated O/N with 50 ng rh DcR3:Fc (Prod. No.
ALX-522-055) per well. After blocking, the indicated concentrations of rhsFasL (Prod. No.
ALX-522-001), rhs
SuperFasL (Prod. No.
ALX-522-020) or rhsLIGHT (Prod. No.
ALX-522-018), were added for 1 hour. After extensive washing, enhancer for ligands (Prod. No.
ALX-804-034) at 1µg/ml was added and incubated for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP (1:1000 dilution, Jackson Lab.) incubated for 1 hour. OPD was used as a substrate, and absorbance was measured at 490 nm in an ELISA reader. "
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Product Literature References
A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis: K.Y. Yu, et al.; J. Biol. Chem.
274, 13733 (1999),
Abstract;
Full Text
Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer: R.M. Pitti, et al.; Nature
396, 699 (1998),
Abstract;
General Literature References
Modulation of dendritic cell differentiation and maturation by decoy receptor 3: T.L. Hsu, et al.; J. Immunol.
168, 4846 (2002),
Abstract;
Soluble decoy receptor 3 is expressed by malignant gliomas and suppresses CD95 ligand-induced apoptosis and chemotaxis: W. Roth, et al.; Cancer Res.
61, 2759 (2001),
Abstract;
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