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BAFF (soluble) (mouse), (recombinant)

 
ALX-522-052-C010 10 µg 395.00 USD
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Product Specification

Alternative Name:BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
 
MW:~17kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG®-tag.
 
UniProt ID:Q9WU72
 
Gene/Protein Identifier:A000383 (UCSD Signaling Gateway ID)
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Specificity:Binds to human (weak) and mouse BCMA, TACI and BAFF-R.
 
Applications:ELISA
 
Application Notes:ELISA: binds to BAFF receptor at 1-100 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that BAFF mediates splenocyte survival.

FLAG is a registered trademark of Sigma-Aldrich Co.
 
522-052 Graph
Figure: BAFF, Soluble (mouse) (rec.) mediates survival of freshly isolated splenocytes. Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of BAFF (Prod. No. ALX-522-052) as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.
522-052 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1 μg BAFF, stained with Imperial stain
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522-052 Graph 522-052 SDS-PAGE

Product Literature References

Deleting the BAFF receptor TACI protects against systemic lupus erythematosus without extensive reduction of B cell numbers: W.A. Figgett, et al.; J. Autoimmun. 61, 9 (2015), Application(s): In Vitro Cultures, Abstract;
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005), Abstract;
Act1, a negative regulator in CD40- and BAFF-mediated B cell survival: Y. Qian, et al.; Immunity 21, 575 (2004), Abstract;

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