Product Details
| Alternative Name: | BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257 |
| |
| MW: | ~17kDa (SDS-PAGE). |
| |
| Source: | Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG®-tag. |
| |
| UniProt ID: | Q9WU72 |
| |
| Gene/Protein Identifier: | A000383 (UCSD Signaling Gateway ID) |
| |
| Concentration: | 0.1mg/ml after reconstitution. |
| |
| Formulation: | Lyophilized. Contains PBS. |
| |
| Purity: | ≥90% (SDS-PAGE) |
| |
| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
| |
| Specificity: | Binds to human (weak) and mouse BCMA, TACI and BAFF-R. |
| |
| Applications: | ELISA
|
| |
| Application Notes: | ELISA: binds to BAFF receptor at 1-100 ng/ml. |
| |
| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Technical Info/Product Notes: | Historical data has shown that BAFF mediates splenocyte survival.
FLAG is a registered trademark of Sigma-Aldrich Co. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: BAFF, Soluble (mouse) (rec.) mediates survival of freshly isolated splenocytes. Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of BAFF (Prod. No. ALX-522-052) as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.
SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1 μg BAFF, stained with Imperial stain
Please mouse over
Product Literature References
Deleting the BAFF receptor TACI protects against systemic lupus erythematosus without extensive reduction of B cell numbers: W.A. Figgett, et al.; J. Autoimmun.
61, 9 (2015),
Application(s): In Vitro Cultures,
Abstract;
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med.
202, 1363 (2005),
Abstract;
Act1, a negative regulator in CD40- and BAFF-mediated B cell survival: Y. Qian, et al.; Immunity
21, 575 (2004),
Abstract;
