BAFF (soluble) (mouse), (recombinant)



ALX-522-052-C010	10 µg	354.00 USD

Product Specification

Alternative Name:	BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~17kDa (SDS-PAGE).

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Mediates splenocyte survival.

Source/Host:	Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.

Specificity:	Binds to human (weak) and mouse BCMA, TACI and BAFF-R.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q9WU72: BAFF (mouse) AfCS Signaling Gateway ID A000383: BAFF (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure: BAFF, Soluble (mouse) (rec.) mediates survival of freshly isolated splenocytes. Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of BAFF (Prod. No. ALX-522-052) as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.

SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1 μg BAFF, stained with Imperial stain

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Product Literature References

BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005), Abstract;

Act1, a negative regulator in CD40- and BAFF-mediated B cell survival: Y. Qian, et al.; Immunity 21, 575 (2004), Abstract;