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LTβR (human):Fc (human), (recombinant)

 
ALX-522-041-C050 50 µg 398.00 USD
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Product Specification

Alternative Name:TNFRSF 3, Tumor necrosis factor receptor 2-related protein
 
MW:Migrates as a doublet at ~56kDa and ~60kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The cysteine-rich region of human LTβR (aa 1-220) is fused to the Fc portion of human IgG1.
 
UniProt ID:P36941
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity Detail:Purified by single-step affinity chromatography.
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse LIGHT and LTα1β2.
 
Applications:ELISA
 
Application Notes:ELISA: binds to LIGHT at 20-500 ng/ml.
Detection of human membrane-bound LIGHT in combination with PAb to human IgG1.
 
Reconstitution:Reconstitute in 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:LTβR is a 75kDa type I transmembrane protein belonging to the TNF receptor family. It is 414 aa long and contains 4 cysteine-rich repeats in its extracellular domain. It is expressed on monocytes, fibroblasts, stromal and muscle cells. It is known to activate nuclear factor NF-κB, to interact with NIK and to induce cell death via TNF receptor-associated factor 3 (TRAF3). LTβR has two known ligands: LIGHT and LTα1β2. It has been shown to be involved in splenic architecture development, lymph node genesis and affinity maturation, as well as in the development of diabetes and colitis. It has been implicated in the induction of HIV replication and impairs prion replication in mice lacking follicular dendritic cells. Historical data has shown that LTβR inhibits human LIGHT- and LTα1β2-mediated effects, such as cytotoxic effects, on HT29 target cells.
 

Product Literature References

Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human: C. Bossen, et al.; J. Biol. Chem. 281, 13964 (2006), Abstract; Full Text
Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005), Abstract;
The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem. 275, 14307 (2000), Abstract; Full Text

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