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CD30L (soluble) (human), (recombinant)

 
ALX-522-028-C010 10 µg 218.00 USD
 
ALX-522-028-3010 3x10 µg SuperPack 457.00 USD
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Product Specification

Alternative Name:CD153, TNFSF 8
 
MW:~35kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of recombinant human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.
 
UniProt ID:P32971
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Specificity:Binds to human CD30 (Ki-1).
 
Applications:ELISA
 
Application Notes:ELISA: binds to CD30 receptor.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:The CD30 ligand (CD30L, CD153) is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases. Historical data has shown that CD30L can be used together with an enhancer (Prod. No. ALX-804-034) to detect membrane-bound human CD30. rhsCD30L has also been shown to saturate its receptor at a concentration range of 12-4000ng/ml.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 

Product Literature References

Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004), Abstract;
Selective enrichment of CD30-expressing cells within the blast region of lymphocytes from patients with primary HIV infection (PHI): P. Biswas, et al.; J. Biol. Regul. Homeost. Agents 16, 33 (2002), Abstract;

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