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CD134 (human):Fc (human), (recombinant)

 
ALX-522-022-C050 50 µg 409.00 USD
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Product Specification

Alternative Name:OX40, TNFRSF 4
 
MW:~72kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The cysteine-rich region of human CD134 (OX40) (aa 29-214) is fused to the Fc portion of human IgG1.
 
UniProt ID:P43489
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse CD134L (OX40L).
 
Applications:ELISA
 
Application Notes:ELISA: binds to CD134L).
Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD134L. Has been used to ameliorate graft-versus-host disease, experimental allergic encephalomyelitis and inflammatory bowel disease.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Scientific Background:The CD134 receptor (OX40) is a member of the tumor necrosis factor receptor (TNF-R) family. CD134 is a type I transmembrane receptor. It contains 3 extracellular cysteine-rich motifs. CD134 is mainly expressed in activated lymphocytes. After interaction with its ligand CD134L (OX40L), CD134 interacts with TNF receptor-associated factor 2 (TRAF2), TRAF5 and mediate activation of nuclear factors NF-κB and AP1. CD134-CD134L have been reported to be involved in T cell selection, migration and induction of Th1 and Th2 cytokine pattern. Moreover, it is involved in anti-tumor immunity, cell adhesion and various auto-immune diseases. Historical data has shown that CD134 receptor blocks T cell proliferation induced by CD134L.
 
522-022
Figure: Receptor binding assay. Dose-dependent binding of recombinant human soluble CD134L to recobinant human CD134:Fc. Recombinant human CD134L saturates its receptor at a concentration range of 500-1’000ng/ml.Method: Receptor binding assay: A 96 well ELISA plate was coated over night with 50ng CD134 (human):Fc (human) (recombinant) (Prod. No. ALX-522-022) per well. After a blocking step, the indicated concentrations of CD134L, Soluble (human) (recombinant) (FLAG®) (Prod. No. ALX-522-023) were added for 1 hour. After extensive washing, Enhancer for Ligands (Prod. No. ALX-804-034) was added for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP incubated for 1 hour. OPD was used as a substrate, absorbance was measured at 490nm in an ELISA reader.
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522-022

Product Literature References

Molecular transfer of CD40 and OX40 ligands to leukemic human B cells induces expansion of autologous tumor-reactive cytotoxic T lymphocytes: E. Biagi, et al.; Blood 105, 2436 (2005), Abstract;

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