CD134 (human):Fc (human), (recombinant)



ALX-522-022-C050	50 µg	356.00 USD

Product Specification

Alternative Name:	OX40, TNFRSF 4

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~72kDa (SDS-PAGE).

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Blocks T cell proliferation induced by CD134L.

Source/Host:	Produced in HEK 293 cells. The cysteine-rich region of human CD134 (OX40) (aa 29-214) is fused to the Fc portion of human IgG1.

Reconstitution:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Specificity:	Binds human and mouse CD134L (OX40L).

Application:	Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD134L. Has been used to ameliorate graft-versus-host disease, experimental allergic encephalomyelitis and inflammatory bowel disease.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The CD134 receptor (OX40) is a member of the tumor necrosis factor receptor (TNF-R) family. CD134 is a type I transmembrane receptor. It contains 3 extracellular cysteine-rich motifs. CD134 is mainly expressed in activated lymphocytes. After interaction with its ligand CD134L (OX40L), CD134 interacts with TNF receptor-associated factor 2 (TRAF2), TRAF5 and mediate activation of nuclear factors NF-κB and AP1. CD134-CD134L have been reported to be involved in T cell selection, migration and induction of Th1 and Th2 cytokine pattern. Moreover, it is involved in anti-tumor immunity, cell adhesion and various auto-immune diseases.

Background / Technical Information:	UniProt ID P43489: CD134 (human)

Figure: Receptor binding assay. Dose-dependent binding of recombinant human soluble CD134L to recobinant human CD134:Fc. Recombinant human CD134L saturates its receptor at a concentration range of 500-1’000ng/ml.Method: Receptor binding assay: A 96 well ELISA plate was coated over night with 50ng CD134 (human):Fc (human) (recombinant) (Prod. No. ALX-522-022) per well. After a blocking step, the indicated concentrations of CD134L, Soluble (human) (recombinant) (FLAG^®) (Prod. No. ALX-522-023) were added for 1 hour. After extensive washing, Enhancer for Ligands (Prod. No. ALX-804-034) was added for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP incubated for 1 hour. OPD was used as a substrate, absorbance was measured at 490nm in an ELISA reader.

Please mouse over

Product Literature References

Molecular transfer of CD40 and OX40 ligands to leukemic human B cells induces expansion of autologous tumor-reactive cytotoxic T lymphocytes: E. Biagi, et al.; Blood 105, 2436 (2005), Abstract;