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TWEAK (soluble) (human), (recombinant)

 
ALX-522-021-C010 10 µg 388.00 USD
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Product Specification

Alternative Name:Apo-3L, TNFSF 12
 
MW:~18-20kDa (SDS-PAGE).
 
Source:Produced in E. coli. The extracellular domain of human TWEAK (aa 106-249) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.
 
UniProt ID:O43508
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse Fn14 (TWEAK-R).
 
Biological Activity:Kills TWEAK sensitive cells.
 
Applications:ELISA
 
Application Notes:ELISA: binds to FN14 receptor at 10-100 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that TWEAK has a pro-apoptotic effect on several target cell lines, and it induces angiogenesis and proliferation in endothelial cells.

FLAG is a registered trademark of Sigma-Aldrich Co.
 
522-036
Figure: Apoptosis of TWEAK sensitive cells. Recombinant, human TWEAK exerts its full biological activity in a concentration range of 50-500ng/ml on Kym-1 cells.Method: Kym-1 cells (20’000 cells in 50µl RPMI medium containing 10% fetal calf serum) were incubated with the indicated concentrations of rhsTWEAK for 24 hours at 37°C in a 96 well plate. Concentrations of rhsTWEAK required to kill target cells may vary depending on cell type studied. Cell viability was determined using a PMS/MTS-based cell proliferation assay kit.
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522-036

Product Literature References

Non-canonical NFκB activation promotes chemokine expression in podocytes: L. Valino-Rivas, et al.; Sci. Rep. 6, 28857 (2016), Application(s): Cell cultures, renal cells, Abstract; Full Text
Intraovarian TWEAK/Fn14 ligand-receptor system limits ovarian preovulatory follicles from excessive luteinization: A. De, et al.; Mol. Endocrinol. 20, 2528 (2006), Abstract; Full Text
Induction of RANTES by TWEAK/Fn14 interaction in human keratinocytes: L. Jin, et al.; J. Invest. Dermatol. 122, 1179 (2004), Abstract;
TWEAK/Fn14 interaction stimulates human bronchial epithelial cells to produce IL-8 and GM-CSF: H. Xu, et al.; BBRC 318, 422 (2004), Abstract;

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