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LIGHT (soluble) (human), (recombinant)

 
ALX-522-018-C010 10 µg 400.00 USD
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Product Specification

Alternative Name:HVEML, TNFSF 14, CD258
 
MW:~25kDa (SDS-PAGE)
 
Source:Produced in HEK 293 cells. The extracellular domain of human LIGHT (aa 89-240) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG®-tag.
 
UniProt ID:O43557
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse LTβR and human HVEM and DcR3.
 
Applications:ELISA
 
Application Notes:ELISA: binds to HVEM receptor at 1-10 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data shows that the biological activity of LIGHT increases LNCaP (prostate cancer cell) growth.

FLAG is a registered trademark of Sigma-Aldrich Co.
 
522-018
Figure: Binding of recombinant human DcR3:Fc to soluble human recombinant LIGHT.
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522-018

Product Literature References

Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFκB Signaling: S.F. Dwyer, et al.; Int. J. Biol. Sci. 11, 404 (2015), Application(s): Cell Culture, Abstract; Full Text
LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al.; BBRC 359, 379 (2007), Abstract;
A coreceptor interaction between the CD28 and TNF receptor family members B and T lymphocyte attenuator and herpesvirus entry mediator: L.C. Gonzalez, et al.; PNAS 102, 1116 (2005), Abstract; Full Text
Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin beta receptor reveals a novel mechanism of apoptosis: J. Kuai, et al.; J. Biol. Chem. 278, 14363 (2003), Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001), Abstract;

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