Product Details
| Alternative Name: | HVEML, TNFSF 14, CD258 |
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| MW: | ~25kDa (SDS-PAGE) |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human LIGHT (aa 89-240) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG®-tag. |
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| UniProt ID: | O43557 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse LTβR and human HVEM and DcR3. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to HVEM receptor at 1-10 ng/ml. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data shows that the biological activity of LIGHT increases LNCaP (prostate cancer cell) growth.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Binding of recombinant human DcR3:Fc to soluble human recombinant LIGHT.
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Product Literature References
Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFκB Signaling: S.F. Dwyer, et al.; Int. J. Biol. Sci.
11, 404 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al.; BBRC
359, 379 (2007),
Abstract;
A coreceptor interaction between the CD28 and TNF receptor family members B and T lymphocyte attenuator and herpesvirus entry mediator: L.C. Gonzalez, et al.; PNAS
102, 1116 (2005),
Abstract;
Full Text
Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin beta receptor reveals a novel mechanism of apoptosis: J. Kuai, et al.; J. Biol. Chem.
278, 14363 (2003),
Abstract;
Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol.
167, 6225 (2001),
Abstract;
