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CD40 (human):Fc (human), (recombinant)

 
ALX-522-016-C050 50 µg 370.00 USD
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Product Specification

Alternative Name:TNFRSF 5
 
MW:~54kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.
 
UniProt ID:P25942
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse CD40L (CD154).
 
Applications:ELISA
 
Application Notes:ELISA: binds to CD40L at 1-10 ng/ml.
Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
522-016
Figure: Jurkat T cells clone D1.1 expresses high surface levels of CD40L. Cells were immunostained at 4°C for 30 minutes with 10µg/ml of CD40:Fc (Prod. No ALX-522-016). After washing, cells were incubated  for 30 min.  at 4°C with 10µg/ml goat anti-human RPE (Prod. No ALX-211-501). After washing, cells were analyzed by Flow Cytometry.
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522-016

Product Literature References

Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000), Abstract;

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