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CD40L (soluble) (human), (recombinant)

Increases the stimulation of B cells
 
ALX-522-015-C010 10 µg 163.00 USD
 
ALX-522-015-2010 2x10 µg 222.00 USD
 
ALX-522-015-6010 6x10 µg SuperPack 543.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:CD154, TNFSF 5, gp39
 
MW:~18kDa (SDS-PAGE).
 
Source:Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
 
UniProt ID:P29965
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by Multi-Step Chromatography.
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Specificity:Binds to human CD40.
 
Applications:ELISA
 
Application Notes:ELISA: binds to CD40 receptor at 1-10 ng/ml.
 
Reconstitution:Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that CD40L stimulates growth of B cells. The activity of rhsCD40L increases 1'000-fold (stimulation in the ng/ml range) in the presence of cross-linking enhancer (see Set Prod. No. ALX-850-064). For stimulation of mouse cells via CD40 use CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070 or ALX-850-075).

FLAG is a registered trademark of Sigma-Aldrich Co.
 
522-015-1
Schematic structure of human CD40L (peptide, aa. 116 to 261).
522-015-2
Figure 2: Stimulation of B cells by CD40L, Soluble (human) (rec.). Dose dependent costimulation of CD19+ cells by CD40L in the presence of 10ng/ml of IL-4 and of 2µg/ml anti-µ antibodies. Note that in the presence of enhancer (Prod. No. ALX-804-034), the activity of CD40L is increased >1’000 fold.Method: CD19+ cells were incubated in 96-well plates (105 cells/well in 100µl RPMI supplemented with 10% FCS) for 72 hours in the presence of 10ng/ml IL-4, 2µg/ml of goat anti-human µ chain antibody and with the indicated concentration of CD40L in the presence and absence of 1µg/ml enhancer (Prod. No. ALX-804-034). Cells were pulsed for an additional 6 hours with [3H]thymidine (1µCi/well) and harvested. [3H]thymidine incorporation was monitored by liquid scintillation counting.
522-015 SDS-PAGE
SDS-PAGE Analysis of CD40L (soluble) (human), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg.
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522-015-1 522-015-2 522-015 SDS-PAGE

Product Literature References

B-cell activation with CD40L or CpG measures the function of B-cell subsets and identifies specific defects in immunodeficient patients: E. Marasco, et al.; Eur. J. Immunol. (2016), Application(s): Human PBMC culture, Abstract; Full Text
CD40 signaling in Graves' disease is mediated through canonical and non-canonical thyroidal NF-κB activation: H.J. Lee, et al.; Endocrinology (2016), Abstract;
Identification and characterization of agonist epitopes of the MUC1-C oncoprotein: C. Jochems, et al.; Cancer Immunol. Immunother. 63, 161 (2014), Application(s): Stimulation of dendritic cells, Abstract;
Immunological targeting of tumor cells undergoing an epithelial-mesenchymal transition via a recombinant brachyury-yeast vaccine: D.H. Hamilton, et al.; Oncotarget 4, 1777 (2013), Application(s): Stimulation of dendritic cells, Abstract; Full Text
Suppression by Δ9-tetrahydrocannabinol of the Primary Immunoglobulin M Response by Human Peripheral Blood B cells is Associated with Impaired STAT3 Activation: T. Ngaotepprutaram, et al.; Toxicology 310, 84 (2013), Application(s): Flow Cytometry, Abstract; Full Text
Elevated serum soluble CD40 ligand in cancer patients may play an immunosuppressive role: J. Huang, et al.; Blood 120, 3030 (2012), Application(s): Cell Culture, Abstract; Full Text
Leukotriene C4 induces migration of human monocyte-derived dendritic cells without loss of immunostimulatory function: J. Dannull, et al.; Blood 119, 3113 (2012), Application(s): Stimulation of dendritic cells, Abstract; Full Text
Targeting HIV-1 Envelope Glycoprotein Trimers to B Cells by Using APRIL Improves Antibody Responses: M. Melchers, et al.; J. Virol. 86, 2488 (2012), Application(s): Cell Culture, Abstract; Full Text
The death domain kinase RIP1 links the immunoregulatory CD40 receptor to apoptotic signaling in carcinomas: P. G. Knox, et al.; J. Cell Biol. 192, 391 (2011), Abstract; Full Text
Adenovirus delivery of human CD40 ligand gene confers direct therapeutic effects on carcinomas: L. Vardouli, et al.; Cancer Gene Ther. 16, 848 (2009), Application(s): Cell Culture, Abstract;
C4b Binding Protein Binds to CD154 Preventing CD40 Mediated Cholangiocyte Apoptosis: A Novel Link between Complement and Epithelial Cell Survival: K.T. Williams, et al.; PLoS ONE 2, e159 (2007), Application(s): Cell Culture, Abstract; Full Text
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004), Abstract;
HIV-1 Nef intersects the macrophage CD40L signalling pathway to promote resting-cell infection: S. Swingler, et al.; Nature 424, 213 (2003), Abstract; Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003), Abstract;
Pro-inflammatory effect of TWEAK/Fn14 interaction on human umbilical vein endothelial cells: N. Harada, et al.; BBRC 299, 488 (2002), Abstract;
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002), Abstract;
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002), Abstract;
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001), Abstract; Full Text
Histamine polarizes human dendritic cells into Th2 cell-promoting effector dendritic cells: G. Caron, et al.; J. Immunol. 167, 3682 (2001), Abstract;
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000), Abstract; Full Text
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 12051 (1998), Abstract; Full Text

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